Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

Wenzhong Yang; Seungrag Lee; Jiyong Lee; Yoonsung Bae; Dugyoung Kim

doi:10.1117/12.842925

17 February 2010 Silver nanoparticle-induced degranulation observed with quantitative phase microscopy

Wenzhong Yang, Seungrag Lee, Jiyong Lee, Yoonsung Bae, Dugyoung Kim

Proceedings Volume 7575, Colloidal Quantum Dots for Biomedical Applications V; 757515 (2010) https://doi.org/10.1117/12.842925
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The use of AgNP is becoming more and more widespread in biomedical field. But compared with the promising bactericidal function, other physiological effects of AgNP on cells are relatively scant. In this research, we propose quantitative phase microscopy (QPM) as a new method to study the degranulation, and AgNP-induced RBL-2H3 cell degranulation is studied as well. Firstly, HeLa cells as the cell control and PBS as the solvent control, we measured the cell volume and cross section profile (x-z plane) with QPM. The results showed that the volume and cross section profile changed only the RBL-2H3 cells exposed to calcium ionophore A23187, which demonstrates the validity of QPM in degranulation research. Secondly, 50μg/mL of AgNP was used instead of A23187, and the measurement of cell volume and cross section profile was carried out again. RBL-2H3 cell volume increased immediately after AgNP was added, and cross section profile showed that the cell surface became granulated, but HeLa cell was lack of that effect. Phase images obviously indicated the RBL-2H3 cell deformation. Thirdly, stained with Fluo-3/AM, intracellular calcium Ca²⁺]_i of single RBL-2H3 cell treated with AgNP was observed with fluorescent microscopy; incubated with AgNP for 20min, the supernatant of RBL-2H3 cells was collected and reacted with o-phthalaldehyde (OPA), then the fluorescent intensity of histamine-OPA complex was assayed with spectrofluorometer. The results of Ca²⁺]_i and histamine increase showed that degranulation of AgNP-induced RBL-2H3 cell occurred. So, the cell volume was used as a parameter of degranulation in our study and AgNP-induced RBL-2H3 cells degranulation was confirmed by the cell volume increment, cross section profile change, and [Ca²⁺]_i and histamine in supernatant increase.

Citation Download Citation

Wenzhong Yang, Seungrag Lee, Jiyong Lee, Yoonsung Bae, and Dugyoung Kim "Silver nanoparticle-induced degranulation observed with quantitative phase microscopy", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 757515 (17 February 2010); https://doi.org/10.1117/12.842925

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopy

Calcium

Luminescence

Silver

Biomedical optics

Charge-coupled devices

Digital image correlation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years