Sample to answer: a fully integrated nucleic acid identification system for bacteria monitoring

Jungkyu Kim; John Elsnab; Michael Johnson; Bruce K. Gale

doi:10.1117/12.844856

17 February 2010 Sample to answer: a fully integrated nucleic acid identification system for bacteria monitoring

Jungkyu Kim, John Elsnab, Michael Johnson, Bruce K. Gale

Proceedings Volume 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII; 75930S (2010) https://doi.org/10.1117/12.844856
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

A fully integrated microfluidic system was developed and incorporates an EC-MWCNT (electrochemical multiwalled carbon nanotube) sensor for the detection of bacteria. Sample metering, reagent metering and delivery was implemented with microvalves and pumps embedded inside the microfluidic system. The nucleic acid extraction was performed using microchannels controlled using automated platforms and a disposable microfluidic silica cartridge. The target samples were flowed and hybridized with probe ssDNA (single strand DNA) across the MWCNT-EC sensor (built on a silicon chip), which was embedded in a microfluidic cell. The 9-pad sensor was scanned before and after hybridization to measure the quantity of RNA (Ribonucleic acid) bound to the array surface. A rapid and accurate sample-in answer-out nucleic acid system was realized with automated volume metering, microfluidic sample preparation, and integrated nano-biosensors.

Citation Download Citation

Jungkyu Kim, John Elsnab, Michael Johnson, and Bruce K. Gale "Sample to answer: a fully integrated nucleic acid identification system for bacteria monitoring", Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930S (17 February 2010); https://doi.org/10.1117/12.844856

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available