A novel fiber optic biosensor for nitric oxide determination based on vicinal diaminobenzozcridine fluorescent probe

Liyun Ding; Lanfen Huang; Jun Huang; Yunming Zhong; Dian Fan

doi:10.1117/12.849847

24 April 2010 A novel fiber optic biosensor for nitric oxide determination based on vicinal diaminobenzozcridine fluorescent probe

Liyun Ding, Lanfen Huang, Jun Huang, Yunming Zhong, Dian Fan

Author Affiliations +

Proceedings Volume 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII; 76730Z (2010) https://doi.org/10.1117/12.849847
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

A novel fiber optic biosensor for the determination of nitric oxide based on vicinal diaminobenzozcridine (VDABA) fluorescent probe was designed and fabricated. The reaction conditions between VDABA and NO, which include concentration of VDABA, temperature and pH, were studied in-depth. The sensitivity of VDABA for NO detection under the optimum conditions and its optical properties were also investigated. The fluorescence responses were concentration-dependent and a good linear relationship (R²=0.9863) was observed over the range 1.8×10^-6 to 9×10^-6 mol/L NO, the regression equation was F = 3.8889[NO] (mol/L)+217.2. Besides, a complex sensitive film embedding VDABA in cellulose acetate (CA) was prepared, and a fiber optic NO biosensor was fabricated using this film. Then the change of fluorescence phase shift of this biosensor was studied preliminarily by means of the lock-in technology.

Citation Download Citation

Liyun Ding, Lanfen Huang, Jun Huang, Yunming Zhong, and Dian Fan "A novel fiber optic biosensor for nitric oxide determination based on vicinal diaminobenzozcridine fluorescent probe", Proc. SPIE 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII, 76730Z (24 April 2010); https://doi.org/10.1117/12.849847

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