Nonadiabatic tapered optical fiber sensor for measuring interaction nicotine with DNA

M. I. Zibaii; H. Latifi; H. Pourbeyram; M. Gholami; Z. Taghipour; Z. Saeedian; S. M. Hosseini

doi:10.1117/12.885074

17 May 2011 Nonadiabatic tapered optical fiber sensor for measuring interaction nicotine with DNA

M. I. Zibaii, H. Latifi, H. Pourbeyram, M. Gholami, Z. Taghipour, Z. Saeedian, S. M. Hosseini

Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77536C (2011) https://doi.org/10.1117/12.885074
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada

Abstract

A nonadiabatic tapered optical fiber sensor was utilized for studying of bimolecular interactions including DNA-DNA and DNA-Drug interaction. This work presents a simple evanescent wave sensing system based on an interferometric approach, suitable to meet the requirements of lable-free sensor systems for detecting biomolecular interactions. We have demonstrated the measuring refractive index and the real time detection of interactions between biomolecules. Furthermore basic experiments were carried out, for detecting the hybridization of 25-mer DNA with an immobilized counterpart on the surface. The overall shift after the successful DNA hybridization was 9.5 nm. In this work, a new approach for studying DNA-drug interactions was successfully tested. Nicotine as a carcinogenic compound in cigarette smoke plays an important role in interaction with DNA. Different concentrations of nicotine were applied to observe the Longmuir interaction with DNA.

Citation Download Citation

M. I. Zibaii, H. Latifi, H. Pourbeyram, M. Gholami, Z. Taghipour, Z. Saeedian, and S. M. Hosseini "Nonadiabatic tapered optical fiber sensor for measuring interaction nicotine with DNA", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536C (17 May 2011); https://doi.org/10.1117/12.885074

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