In-vivo optical investigation of psoriasis

Dimitrios Kapsokalyvas; Riccardo Cicchi; Nicola Bruscino; Domenico Alfieri; Daniela Massi; Torello Lotti; Francesco S. Pavone

doi:10.1117/12.874723

17 February 2011 In-vivo optical investigation of psoriasis

Dimitrios Kapsokalyvas, Riccardo Cicchi, Nicola Bruscino, Domenico Alfieri, Daniela Massi, Torello Lotti, Francesco S. Pavone

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Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 78830X (2011) https://doi.org/10.1117/12.874723
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Psoriasis is an autoimmune disease of the skin characterized by hyperkeratosis, hyperproliferation of the epidermis, inflammatory cell accumulation and increased dilatation of dermal papillary blood vessels. Cases of psoriasis were investigated in vivo with optical means in order to evaluate the potential of in vivo optical biopsy. A Polarization Multispectral Dermoscope was employed for the macroscopic observation. Features such as the 'dotted' blood vessels pattern was observed with high contrast. The average size of dot vessels in Psoriasis was measured to be 974 μm² which is much higher compared to healthy skin. High resolution image sections of the epidermis and the dermis were produced with a custom made Multiphoton Microscope. Imaging extended from the surface of the lesion down to the papillary dermis, at a depth of 200 μm. In the epidermis, a characteristic morphology of the stratum corneum found only in Psoriasis was revealed. Additionally, the cytoplasmic area of the cells in the stratum spinosum layer was found to be smaller than normal. In the dermis the morphological features were more pronounced, where the elongated dermal papillae dominated the papillary layer. Their length exceeds 100μm, which is a far greater value compared to that of healthy skin. These in vivo observations are consistent with the ex vivo histopathological observations, supporting both the applicability and potentiality of multispectral dermoscopy and multiphoton microscopy in the field of in vivo optical investigation and biopsy of skin.

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Dimitrios Kapsokalyvas, Riccardo Cicchi, Nicola Bruscino, Domenico Alfieri, Daniela Massi, Torello Lotti, and Francesco S. Pavone "In-vivo optical investigation of psoriasis", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830X (17 February 2011); https://doi.org/10.1117/12.874723

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KEYWORDS

Skin

Blood vessels

Microscopes

In vivo imaging

Head

Polarization

Tissue optics

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