Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo

Hans Georg Breunig; Karsten König

doi:10.1117/12.874990

17 February 2011 Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo

Hans Georg Breunig, Karsten König

Author Affiliations +

Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 788311 (2011) https://doi.org/10.1117/12.874990
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We performed multiphoton imaging of human skin and recorded in combination the complete spectral content of the signals in vivo. The spectra represent the integration of multiphoton signals over the investigated regions of the epidermis and dermis. They are used to study depth-resolved in vivo emission characteristics of main endogenous skin fluorophores like keratin, NAD(P)H, collagen and elastin. The identification of the specific fluorophores is supported by analysis of additional in vivo fluorescence lifetime imaging. Furthermore, as a potential application of spectrally selective imaging the possibility to investigate the penetration of nanoparticles from sunscreen lotion into skin in vivo is discussed.

Citation Download Citation

Hans Georg Breunig and Karsten König "Spectral characteristics of two-photon autofluorescence and second harmonic generation from human skin in vivo", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788311 (17 February 2011); https://doi.org/10.1117/12.874990

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available