Fluorescence enhancement in a polymer-based photonic-crystal biosensor

Bashar Hamza; Anand Kadiyala; Caroline Kilemi; Yuxin Liu; Jeremy Dawson

doi:10.1117/12.874196

8 February 2011 Fluorescence enhancement in a polymer-based photonic-crystal biosensor

Bashar Hamza, Anand Kadiyala, Caroline Kilemi, Yuxin Liu, Jeremy Dawson

Proceedings Volume 7888, Frontiers in Biological Detection: From Nanosensors to Systems III; 788804 (2011) https://doi.org/10.1117/12.874196
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Detecting labeled or naturally-fluorescent biomolecules at very low concentrations is of a significant importance for health sciences, agricultural sciences, and security-related applications. Photonic crystals (PhC) are microfabricated nano-structures of periodic dielectric permittivity in one, two, or three dimensions that possess unique light manipulation properties. These include the ability to localize electromagnetic waves at particular PhC lattice locations. Ultra-sensitive detection using thin-film PhC structures fabricated in semiconductor materials has been demonstrated in both "active" and "passive" modalities. In the active modality, the adsorption of target molecules to the PhC surface causes a refractive index change that is translated into reflectance or transmission peak shifts. The passive modality demonstrated by our group utilizes the PhC structure to observe enhanced fluorescent emission within resonant defect cavities in a 2D PhC lattice. Integrating these semiconductor-based PhC structures with biocompatible microfluidic channels is a challenging task that can significantly increase the final cost of the sensor system. We demonstrate here soft lithographic nanomolding techniques for polymer-based PhC structures that are easily integrated with microfluidic channels to provide a portable means of biosensing. A TE bandgap of 2.857% for a 2D PhC fabricated in poly(dimethylsiloxane) (PDMS) will allow these lattices to become core structures in PhC-based biosensors incorporating both active and passive modalities. Modeling and initial optical characterization results of the Si- and PDMS-based PhC biosensor will also be presented.

Citation Download Citation

Bashar Hamza, Anand Kadiyala, Caroline Kilemi, Yuxin Liu, and Jeremy Dawson "Fluorescence enhancement in a polymer-based photonic-crystal biosensor", Proc. SPIE 7888, Frontiers in Biological Detection: From Nanosensors to Systems III, 788804 (8 February 2011); https://doi.org/10.1117/12.874196

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available