Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences

Tzu-Ming Liu; Chien-Tai Hsieh; Yu-Shing Chen; Fu-Lien Huang; Hsin-Yi Huang; Wen-Jeng Lee; Chun-Ta Kung; Chi-Kuang Sun

doi:10.1117/12.872690

11 February 2011 Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences

Tzu-Ming Liu, Chien-Tai Hsieh, Yu-Shing Chen, Fu-Lien Huang, Hsin-Yi Huang, Wen-Jeng Lee, Chun-Ta Kung, Chi-Kuang Sun

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79032Z (2011) https://doi.org/10.1117/12.872690
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We demonstrated that the intensity levels and lifetimes of two-photon autofluorescences (2PAF) in human liver tissues can be exploited to diagnose hepatocellular carcinoma (HCC). Excited by an infrared femtosecond laser, we suppressed the two-photon autofluorescences of most endogenous fluorophores and made red autofluorescences more specific to particular molecules in the cryo-sectioned human livers. Using such fluorescence contrast, we found HCC tissues have reduced levels of 2PAF and longer fluorescence lifetime.

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Tzu-Ming Liu, Chien-Tai Hsieh, Yu-Shing Chen, Fu-Lien Huang, Hsin-Yi Huang, Wen-Jeng Lee, Chun-Ta Kung, and Chi-Kuang Sun "Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Z (11 February 2011); https://doi.org/10.1117/12.872690

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