Lens-less holographic microscope with high resolving power and no-distortion

Kunihiro Sato; Osamu Murata

doi:10.1117/12.876563

11 February 2011 Lens-less holographic microscope with high resolving power and no-distortion

Kunihiro Sato, Osamu Murata

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790402 (2011) https://doi.org/10.1117/12.876563
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

A new method is developed for recording and reconstructing a microscopic high-resolution 3-D image with no distortion. Any imaging lens is not located between an object and a CCD in our optical system. An off-axis hologram with a large numerical aperture is recorded at once, and a complex-amplitude in-line hologram is extracted from the recorded off-axis hologram by applying the one-shot digital holography. A small complex-amplitude in-line hologram is generated for the reconstruction of microscopic high-resolution images by dividing the large hologram into a number of small holograms and by superimposing them. A focus-free image with high resolving power and no distortion is reconstructed from the generated complex-amplitude in-line hologram. Resolution higher than 1μm is obtained in the optical experiment, which can be improved up to the wave length. Microscopic high-resolution images with no distortion can also be observed for objects immersed in the liquid or in the solid by using the lens-less holographic microscope.

Citation Download Citation

Kunihiro Sato and Osamu Murata "Lens-less holographic microscope with high resolving power and no-distortion", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790402 (11 February 2011); https://doi.org/10.1117/12.876563

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