Pupil engineering for a confocal reflectance line-scanning microscope

Yogesh G. Patel; Milind Rajadhyaksha; Charles A. DiMarzio

doi:10.1117/12.875688

28 February 2011 Pupil engineering for a confocal reflectance line-scanning microscope

Yogesh G. Patel, Milind Rajadhyaksha, Charles A. DiMarzio

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790416 (2011) https://doi.org/10.1117/12.875688
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Confocal reflectance microscopy may enable screening and diagnosis of skin cancers noninvasively and in real-time, as an adjunct to biopsy and pathology. Current confocal point-scanning systems are large, complex, and expensive. A confocal line-scanning microscope, utilizing a of linear array detector can be simpler, smaller, less expensive, and may accelerate the translation of confocal microscopy in clinical and surgical dermatology. A line scanner may be implemented with a divided-pupil, half used for transmission and half for detection, or with a full-pupil using a beamsplitter. The premise is that a confocal line-scanner with either a divided-pupil or a full-pupil will provide high resolution and optical sectioning that would be competitive to that of the standard confocal point-scanner. We have developed a confocal line-scanner that combines both divided-pupil and full-pupil configurations. This combined-pupil prototype is being evaluated to determine the advantages and limitations of each configuration for imaging skin, and comparison of performance to that of commercially available standard confocal point-scanning microscopes. With the combined configuration, experimental evaluation of line spread functions (LSFs), contrast, signal-to-noise ratio, and imaging performance is in progress under identical optical and skin conditions. Experimental comparisons between divided-pupil and full-pupil LSFs will be used to determine imaging performance. Both results will be compared to theoretical calculations using our previously reported Fourier analysis model and to the confocal point spread function (PSF). These results may lead to a simpler class of confocal reflectance scanning microscopes for clinical and surgical dermatology.

Citation Download Citation

Yogesh G. Patel, Milind Rajadhyaksha, and Charles A. DiMarzio "Pupil engineering for a confocal reflectance line-scanning microscope", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790416 (28 February 2011); https://doi.org/10.1117/12.875688

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