Time-lapsed integrated Raman and angular-scattering microscopy of single cells

Dustin W. Shipp; Andrew J. Berger

doi:10.1117/12.873906

11 February 2011 Time-lapsed integrated Raman and angular scattering microscopy of single cells

Dustin W. Shipp, Andrew J. Berger

Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 79070X (2011) https://doi.org/10.1117/12.873906
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Integrated Raman- and Angular-scatteringMicroscopy (IRAM) combines two light scattering techniques to make chemical and morphological measurements of intact, single cells without the use of external labeling. IRAM has previously demonstrated its ability to differentiate between activated and non-activated CD8+ T cells based on both chemical and morphological differences. Activated cells showed an increase in protein and lipid content as well as an increase in the size and number of 0.5-1.0 μm diameter scatterers (likely lysosomes). Recent improvements to the IRAM system enable studies over an extended period of time. The applications of IRAM to chemical and structural changes of single cells during biological processes and treatments will be discussed.

Citation Download Citation

Dustin W. Shipp and Andrew J. Berger "Time-lapsed integrated Raman and angular scattering microscopy of single cells", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070X (11 February 2011); https://doi.org/10.1117/12.873906

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