Non-radiative excitation fluorescence: driving biology beyond the diffraction limit

Pascale Winckler; Rodolphe Jaffiol; Jérôme Plain

doi:10.1117/12.873846

4 February 2011 Non-radiative excitation fluorescence: driving biology beyond the diffraction limit

Pascale Winckler, Rodolphe Jaffiol, Jérôme Plain

Proceedings Volume 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 790802 (2011) https://doi.org/10.1117/12.873846
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We propose a new method of local illumination based on the activation of glass substrates, enables us to drive fluorescence investigations on biological sample at nanometric scale. This local fluorescence excitation is achieved through a non-radiative energy transfer. We demonstrate the potentiality of our technique through two biological relevant applications: imaging of the cell adhesion points and fluorescence correlation spectroscopy in a sub-diffracted volume (attoliter range).

Citation Download Citation

Pascale Winckler, Rodolphe Jaffiol, and Jérôme Plain "Non-radiative excitation fluorescence: driving biology beyond the diffraction limit", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790802 (4 February 2011); https://doi.org/10.1117/12.873846

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