Sensing of bacteria immobilised under static conditions using long-range surface plasmon waveguides in Cytop

Asad Khan; Oleksiy Krupin; Ewa Lisicka-Skrzek; Pierre Berini

doi:10.1117/12.902474

31 August 2011 Sensing of bacteria immobilised under static conditions using long-range surface plasmon waveguides in Cytop

Asad Khan, Oleksiy Krupin, Ewa Lisicka-Skrzek, Pierre Berini

Author Affiliations +

Proceedings Volume 8007, Photonics North 2011; 800703 (2011) https://doi.org/10.1117/12.902474
Event: Photonics North 2011, 2011, Ottawa, Canada

Abstract

Waveguides consisting of Au embedded in Cytop with micro-fluidic channels etched into the cladding are used for sensing via the propagation of long-range surface plasmons. Initially, a range of water/glycerol solutions with varying refractive indices were sequentially injected in a waveguide section in order to assess its bulk sensitivity and to find a solution supporting a strong high quality mode. Au waveguide surfaces were then functionalized with antibodies against Gram negative bacteria (Anti-Gneg) by first forming a self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (16-MHA) and subsequent conjugation with antibodies through carbodiimide chemistry. E.Coli XL-1 Blue was used as an analyte in static incubations. Wavelength sweeps of 16-MHA covered waveguides were compared against waveguides covered with E-coli. The results indicate that very few bacteria cells are required to obtain a measurable change in output signal.

Citation Download Citation

Asad Khan, Oleksiy Krupin, Ewa Lisicka-Skrzek, and Pierre Berini "Sensing of bacteria immobilised under static conditions using long-range surface plasmon waveguides in Cytop", Proc. SPIE 8007, Photonics North 2011, 800703 (31 August 2011); https://doi.org/10.1117/12.902474

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