Nonlinear optical molecular imaging enables metabolic redox sensing in tissue-engineered constructs

Leng-Chun Chen; William R. Lloyd; Robert H. Wilson; Shiuhyang Kuo; Cynthia L. Marcelo; Stephen E. Feinberg; Mary-Ann Mycek

doi:10.1117/12.889713

14 June 2011 Nonlinear optical molecular imaging enables metabolic redox sensing in tissue-engineered constructs

Leng-Chun Chen, William R. Lloyd, Robert H. Wilson, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, Mary-Ann Mycek

Author Affiliations +

Proceedings Volume 8089, Molecular Imaging III; 80890J (2011) https://doi.org/10.1117/12.889713
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Tissue-engineered constructs require noninvasive monitoring of cellular viability prior to implantation. In a preclinical study on human Ex Vivo Produced Oral Mucosa Equivalent (EVPOME) constructs, nonlinear optical molecular imaging was employed to extract morphological and functional information from intact constructs. Multiphoton excitation fluorescence images were acquired using endogenous fluorescence from cellular nicotinamide adenine dinucleotide phosphate [NAD(P)H] and flavin adenine dinucleotide (FAD). The images were analyzed to report quantitatively on tissue structure and metabolism (redox ratio). Both thickness variations over time and cell distribution variations with depth were identified, while changes in redox were quantified. Our results show that nonlinear optical molecular imaging has the potential to visualize and quantitatively monitor the growth and viability of a tissue-engineered construct over time.

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Leng-Chun Chen, William R. Lloyd, Robert H. Wilson, Shiuhyang Kuo, Cynthia L. Marcelo, Stephen E. Feinberg, and Mary-Ann Mycek "Nonlinear optical molecular imaging enables metabolic redox sensing in tissue-engineered constructs", Proc. SPIE 8089, Molecular Imaging III, 80890J (14 June 2011); https://doi.org/10.1117/12.889713

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