A biophotonic study of live, flowing red blood cells in an optical trap

H. Basu; A. K. Dharmadhikari; J. A. Dharmadhikari; S. Sharma; D. Mathur

doi:10.1117/12.897952

23 August 2011 A biophotonic study of live, flowing red blood cells in an optical trap

H. Basu, A. K. Dharmadhikari, J. A. Dharmadhikari, S. Sharma, D. Mathur

Proceedings Volume 8173, Photonics 2010: Tenth International Conference on Fiber Optics and Photonics; 817309 (2011) https://doi.org/10.1117/12.897952
Event: International Conference on Fiber Optics and Photonics, 2010, Guwahati, India

Abstract

We investigate the physics of an optically trapped red blood cell under physiological conditions. When a single, live red blood cell, is placed in an optical trap, the normal biconcave disk shaped cell is observed to undergo a folding action and thereby take up a rod like shape. If such an RBC has any shape anisotropies due to perturbation through malarial infection or hyperosmotic stress, it is observed to rotate in the linearly polarised laser field. Finally when such an optically trapped RBC is exposed to a shear flow, a tank treading like behaviour of the red blood cell membrane is visualised (wherein the RBC membrane revolves around the central body of the cell). The tank treading motion of a red blood cell held stationary in the optical trap allows for the dynamics to be viewed in a prolonged manner without the usage of earlier constraints such fast imaging system.

Citation Download Citation

H. Basu, A. K. Dharmadhikari, J. A. Dharmadhikari, S. Sharma, and D. Mathur "A biophotonic study of live, flowing red blood cells in an optical trap", Proc. SPIE 8173, Photonics 2010: Tenth International Conference on Fiber Optics and Photonics, 817309 (23 August 2011); https://doi.org/10.1117/12.897952

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KEYWORDS

Optical tweezers

Blood

Motion models

Anisotropy

Absorbance

Biomedical optics

Cytoskeletons

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