Raman spectroscopic imaging as complementary tool for histopathologic assessment of brain tumors

Christoph Krafft; Norbert Bergner; Bernd Romeike; Rupert Reichart; Rolf Kalff; Kathrin Geiger; Matthias Kirsch; Gabriele Schackert; Jürgen Popp

doi:10.1117/12.908668

9 February 2012 Raman spectroscopic imaging as complementary tool for histopathologic assessment of brain tumors

Christoph Krafft, Norbert Bergner, Bernd Romeike, Rupert Reichart, Rolf Kalff, Kathrin Geiger, Matthias Kirsch, Gabriele Schackert, Jürgen Popp

Author Affiliations +

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 82074I (2012) https://doi.org/10.1117/12.908668
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Raman spectroscopy enables label-free assessment of brain tissues and tumors based on their biochemical composition. Combination of the Raman spectra with the lateral information allows grading of tumors, determining the primary tumor of brain metastases and delineating tumor margins - even during surgery after coupling with fiber optic probes. This contribution presents exemplary Raman spectra and images collected from low grade and high grade regions of astrocytic gliomas and brain metastases. A region of interest in dried tissue sections encompassed slightly increased cell density. Spectral unmixing by vertex component analysis (VCA) and N-FINDR resolved cell nuclei in score plots and revealed the spectral contributions of nucleic acids, cholesterol, cholesterol ester and proteins in endmember signatures. The results correlated with the histopathological analysis after staining the specimens by hematoxylin and eosin. For a region of interest in non-dried, buffer immersed tissue sections image processing was not affected by drying artifacts such as denaturation of biomolecules and crystallization of cholesterol. Consequently, the results correspond better to in vivo situations. Raman spectroscopic imaging of a brain metastases from renal cell carcinoma showed an endmember with spectral contributions of glycogen which can be considered as a marker for this primary tumor.

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Christoph Krafft, Norbert Bergner, Bernd Romeike, Rupert Reichart, Rolf Kalff, Kathrin Geiger, Matthias Kirsch, Gabriele Schackert, and Jürgen Popp "Raman spectroscopic imaging as complementary tool for histopathologic assessment of brain tumors", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82074I (9 February 2012); https://doi.org/10.1117/12.908668

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