Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy

Honghai Liu; Wan Qin; Yonghong Shao; Qiuying Liu; Zhen Ma; Thomas K. Borg; Bruce Z. Gao

doi:10.1117/12.908381

22 February 2012 Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy

Honghai Liu, Wan Qin, Yonghong Shao, Qiuying Liu, Zhen Ma, Thomas K. Borg, Bruce Z. Gao

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 822524 (2012) https://doi.org/10.1117/12.908381
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Utilizing a custom-built, on-stage incubator-combined, two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging system, we observed new-sarcomere addition in rat neonatal cardiomyocytes during 10 hours of on-stage incubation. This addition occurred at one end of an existing myofibril, the sides of existing myofibrils, and at the interstice of several separated myofibrils; in the cases of the latter two, we observed mature myofibrils acting as templates. We found that during sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric length during maturation.

Citation Download Citation

Honghai Liu, Wan Qin, Yonghong Shao, Qiuying Liu, Zhen Ma, Thomas K. Borg, and Bruce Z. Gao "Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822524 (22 February 2012); https://doi.org/10.1117/12.908381

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available