Multimodal light-sheet microscopy for fluorescence live imaging

Y. Oshima; H. Kajiura-Kobayashi; S. Nonaka

doi:10.1117/12.907937

2 February 2012 Multimodal light-sheet microscopy for fluorescence live imaging

Y. Oshima, H. Kajiura-Kobayashi, S. Nonaka

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82271H (2012) https://doi.org/10.1117/12.907937
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical sectioning with light-sheet illumination in developmental biology. We have been developed a multifunctional light-sheet fluorescence microscopy system with a near infrared femto-second fiber laser, a high sensitive image sensor and a high throughput spectrometer. We performed that multiphoton fluorescence images of a transgenic fish and a mouse embryo were observed on the light-sheet microscope. As the results, two photon images with high contrast and high spatial resolution were successfully obtained in the microscopy system. The system has multimodality, not only mutiphoton fluorescence imaging, but also hyperspectral imaging, which can be applicable to fluorescence unmixing analysis and Raman imaging. It enables to obtain high specific and high throughput molecular imaging in vivo and in vitro.

Citation Download Citation

Y. Oshima, H. Kajiura-Kobayashi, and S. Nonaka "Multimodal light-sheet microscopy for fluorescence live imaging", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271H (2 February 2012); https://doi.org/10.1117/12.907937

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