Snapshot hyperspectral imaging to measure oxygen saturation in the retina using fiber bundle and multi-slit spectrometer

Bahram Khoobehi; Aurash Khoobehi; Paul Fournier

doi:10.1117/12.910056

2 February 2012 Snapshot hyperspectral imaging to measure oxygen saturation in the retina using fiber bundle and multi-slit spectrometer

Bahram Khoobehi, Aurash Khoobehi, Paul Fournier

Author Affiliations +

Proceedings Volume 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV; 82291E (2012) https://doi.org/10.1117/12.910056
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We have developed a snapshot fiber bundle technique that circumvents the issue of saccades of the non-immobilized eye. In this technology, 458 individual fibers are assembled in a two-dimensional array where each fiber represents a portion of the image. These fibers are redistributed into two separate one-dimensional fiber rows interfaced into a two-slit spectrometer. The light from each fiber is decomposed into its spectral components by the spectrometer. Using this innovative technology, we have been able to detect the whole spectrum of hemoglobin using the single light exposure capabilities of a fundus camera. The hemoglobin signature of the retinal arteries, veins, and retina tissue can be recorded. The final result is a complete, 3-dimensional representation of the spectral and spatial information from a single exposure of the patient. By adjusting the field of view on the imaging portion of the fundus camera, the fiber optic cable may encompass a larger area. However, this causes a decrease in spatial resolution, so we increased the area of the fiber array by increasing the number of the fibers from 458 to 648, increased the size of each individual fiber from 10 μm to 20 &μm, and increased the number of slits to four.

Citation Download Citation

Bahram Khoobehi, Aurash Khoobehi, and Paul Fournier "Snapshot hyperspectral imaging to measure oxygen saturation in the retina using fiber bundle and multi-slit spectrometer", Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82291E (2 February 2012); https://doi.org/10.1117/12.910056

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