Analysis of bacterial growth by UV/Vis spectroscopy and laser reflectometry

Mary Carmen Peña-Gomar; Gonzalo Viramontes-Gamboa; Grethel Peña-Gomar; Mauricio Ortiz Gutiérrez; Mariano Hernández Ramírez

doi:10.1117/12.2001482

24 October 2012 Analysis of bacterial growth by UV/Vis spectroscopy and laser reflectometry

Mary Carmen Peña-Gomar, Gonzalo Viramontes-Gamboa, Grethel Peña-Gomar, Mauricio Ortiz Gutiérrez, Mariano Hernández Ramírez

Author Affiliations +

Proceedings Volume 8412, Photonics North 2012; 84120H (2012) https://doi.org/10.1117/12.2001482
Event: Photonics North 2012, 2012, Montréal, Canada

Abstract

This work presents a preliminary study on an experimental analysis of the lactobacillus bacterial growth in liquid medium with and without the presence of silver nanoparticles. The study aims to quantify the bactericidal effect of nanoparticles. Quantification of bacterial growth at different times was analyzed by spectroscopy UV/visible and laser reflectometry near the critical angle. From these two techniques the best results were obtained by spectroscopy, showing that as the concentration of silver nanoparticles increases, it inhibits the growth of bacteria, it only grows 63% of the population. Regarding Laser Reflectometry, the variation of reflectance near the critical angle is measured in real time. The observed results at short times are reasonable, since they indicate a gradual growth of the bacteria and the stabilization stage of the population. But at long time, the observed results show abrupt changes caused by temperature effects. The bacteria were isolated from samples taken from commercial yougurth, and cultured in MRS broth at pH 6.5, and controlled with citric acid and constant temperature of 32 °C. Separately, silver nanoparticles were synthesized at 3 °C from aqueous solutions of 1.0 mM silver nitrate and chemically reduced with sodium borohydride to 2.0 mM, with magnetic stirring.

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Mary Carmen Peña-Gomar, Gonzalo Viramontes-Gamboa, Grethel Peña-Gomar, Mauricio Ortiz Gutiérrez, and Mariano Hernández Ramírez "Analysis of bacterial growth by UV/Vis spectroscopy and laser reflectometry", Proc. SPIE 8412, Photonics North 2012, 84120H (24 October 2012); https://doi.org/10.1117/12.2001482

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