Non-invasive imaging of zebrafish with spinal deformities using optical coherence tomography: a preliminary study

Liane Bernstein; Kathy Beaudette; Kessen Patten; Émilie Beaulieu-Ouellet; Mathias Strupler; Florina Moldovan; Caroline Boudoux

doi:10.1117/12.2002142

8 March 2013 Non-invasive imaging of zebrafish with spinal deformities using optical coherence tomography: a preliminary study

Liane Bernstein, Kathy Beaudette, Kessen Patten, Émilie Beaulieu-Ouellet, Mathias Strupler, Florina Moldovan, Caroline Boudoux

Author Affiliations +

Proceedings Volume 8565, Photonic Therapeutics and Diagnostics IX; 85656H (2013) https://doi.org/10.1117/12.2002142
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

A zebrafish model has recently been introduced to study various genetic mutations that could lead to spinal deformities such as scoliosis. However, current imaging techniques make it difficult to perform longitudinal studies of this condition in zebrafish, especially in the early stages of development. The goal of this project is to determine whether optical coherence tomography (OCT) is a viable non-invasive method to image zebrafish exhibiting spinal deformities. Images of both live and fixed malformed zebrafish (5 to 21 days postfertilization) as well as wild-type fish (5 to 29 days postfertilization) were acquired non-invasively using a commercial SD-OCT system, with a laser source centered at 930nm (λ=100nm), permitting axial and lateral resolutions of 7 and 8μm respectively. Using two-dimensional images and three-dimensional reconstructions, it was possible to identify the malformed notochord as well as deformities in other major organs at different stages of formation. Visualization of the notochord was facilitated with the development of a segmentation algorithm. OCT images were compared to HE histological sections and images obtained by calcein staining. Because of the possibility of performing longitudinal studies on a same fish and reducing image processing time as compared with staining techniques and histology, the use of OCT could facilitate phenotypic characterization in studying genetic factors leading to spinal deformities in zebrafish and could eventually contribute to the identification of the genetic causes of spinal deformities such as scoliosis.

Citation Download Citation

Liane Bernstein, Kathy Beaudette, Kessen Patten, Émilie Beaulieu-Ouellet, Mathias Strupler, Florina Moldovan, and Caroline Boudoux "Non-invasive imaging of zebrafish with spinal deformities using optical coherence tomography: a preliminary study", Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85656H (8 March 2013); https://doi.org/10.1117/12.2002142

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