Characterizing autofluorescence generated from endogenous porphyrins in cancerous tissue of human colon: case studies

Lina Liu; Lisheng Lin; Weihua Li; Changshun Yang; Zheng Huang; Shusen Xie; Buhong Li

doi:10.1117/12.2002224

19 March 2013 Characterizing autofluorescence generated from endogenous porphyrins in cancerous tissue of human colon: case studies

Lina Liu, Lisheng Lin, Weihua Li, Changshun Yang, Zheng Huang, Shusen Xie, Buhong Li

Author Affiliations +

Proceedings Volume 8577, Optical Biopsy XI; 857703 (2013) https://doi.org/10.1117/12.2002224
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The aim of this case study was to explore the relationship between porphyrins and colon adenocarcinoma, and to examine the potential of porphyrin-induced fluorescence for the diagnosis of colon cancer. Further studies were carried on 8 cases ex vivo colon adenocarcinoma samples which exceptionally exhibited 635 nm fluorescence emission under 405 nm excitation. The time-resolved fluorescence spectra at 635 nm emission under 405 nm excitation were also measured and two-exponential decay fitting was performed to determine the fluorescence lifetime at 635 nm emission. Significant difference was observed between the spectra of normal and cancer tissues, which included an emission peak at 635 nm under the excitation wavelengths of 405 nm. There was also a significant difference between the fluorescence lifetimes of 635 nm emission of the normal tissue and cancer tissue (P<0.05). These results demonstrate that the spectroscopic analysis method allows a selective detection of adenocarcinoma tissues. This spectral profile and lifetime of the red fluorescence resemble that of porphyrins, which suggests that porphyrin fluorescence may be a useful biomarker for characterizing colon cancers of certain patient populations.

Citation Download Citation

Lina Liu, Lisheng Lin, Weihua Li, Changshun Yang, Zheng Huang, Shusen Xie, and Buhong Li "Characterizing autofluorescence generated from endogenous porphyrins in cancerous tissue of human colon: case studies", Proc. SPIE 8577, Optical Biopsy XI, 857703 (19 March 2013); https://doi.org/10.1117/12.2002224

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