An integrated platform for biomolecule interaction analysis

Chia-Ming Jan; Pei-I Tsai; Shin-Ting Chou; Shu-Sheng Lee; Chih-Kung Lee

doi:10.1117/12.2002006

21 February 2013 An integrated platform for biomolecule interaction analysis

Chia-Ming Jan, Pei-I Tsai, Shin-Ting Chou, Shu-Sheng Lee, Chih-Kung Lee

Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 85970F (2013) https://doi.org/10.1117/12.2002006
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We developed a new metrology platform which can detect real-time changes in both a phase-interrogation mode and intensity mode of a SPR (surface plasmon resonance). We integrated a SPR and ellipsometer to a biosensor chip platform to create a new biomolecular interaction measurement mechanism. We adopted a conductive ITO (indium-tinoxide) film to the bio-sensor platform chip to expand the dynamic range and improve measurement accuracy. The thickness of the conductive film and the suitable voltage constants were found to enhance performance. A circularly polarized ellipsometry configuration was incorporated into the newly developed platform to measure the label-free interactions of recombinant human C-reactive protein (CRP) with immobilized biomolecule target monoclonal human CRP antibody at various concentrations. CRP was chosen as it is a cardiovascular risk biomarker and is an acute phase reactant as well as a specific prognostic indicator for inflammation. We found that the sensitivity of a phaseinterrogation SPR is predominantly dependent on the optimization of the sample incidence angle. The effect of the ITO layer effective index under DC and AC effects as well as an optimal modulation were experimentally performed and discussed. Our experimental results showed that the modulated dynamic range for phase detection was 10E-2 RIU based on a current effect and 10E-4 RIU based on a potential effect of which a 0.55 (°/RIU) measurement was found by angular-interrogation. The performance of our newly developed metrology platform was characterized to have a higher sensitivity and less dynamic range when compared to a traditional full-field measurement system.

Citation Download Citation

Chia-Ming Jan, Pei-I Tsai, Shin-Ting Chou, Shu-Sheng Lee, and Chih-Kung Lee "An integrated platform for biomolecule interaction analysis", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 85970F (21 February 2013); https://doi.org/10.1117/12.2002006

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