Endogenous and exogenous fluorescence of gastrointestinal tumors: initial clinical observations

Ekaterina Borisova; Lilia Plamenova; Momchil Keremedchiev; Borislav Vladimirov; Latchezar Avramov

doi:10.1117/12.2016389

15 March 2013 Endogenous and exogenous fluorescence of gastrointestinal tumors: initial clinical observations

Ekaterina Borisova, Lilia Plamenova, Momchil Keremedchiev, Borislav Vladimirov, Latchezar Avramov

Proceedings Volume 8770, 17th International School on Quantum Electronics: Laser Physics and Applications; 87701C (2013) https://doi.org/10.1117/12.2016389
Event: Seventeenth International School on Quantum Electronics: Laser Physics and Applications, 2012, Nessebar, Bulgaria

Abstract

The limitations of standard endoscopy for detection and evaluation of cancerous changes in gastrointestinal tract (GIT) are significant challenge and initiate development of new diagnostic modalities. Therefore many spectral and optical techniques are applied recently into the clinical practice for obtaining qualitatively and quantitatively new data from gastrointestinal neoplasia with different level of clinical applicability and diagnostic success. One of the most promising approaches is fluorescence detection using naturally existing fluorescent molecules or added fluorescent markers. Deltaaminolevulinic acid / protoporphyrin IX is applied for exogenous fluorescent tumor detection in the upper part of gastrointestinal tract. The 5-ALA is administered per os six hours before measurements at dose 20mg/kg weight. Highpower light-emitting diode at 405 nm is used as a source and the excitation light is passed through the light-guide of standard video-endoscopic system to obtain 2-D visualization. Both kinds of spectra – autofluorescence signals and protoporphyrin IX signal are recorded and stored using a fiber-optic microspectrometer, as in endoscopy instrumental channel a fiber is applied to return information about fluorescence signals. In such way 1-D detection and 2-D visualization of the lesions’ fluorescence are received. The results from in vivo detection show significant differentiation between normal and abnormal tissues in 1-D spectroscopic regime, but only moderate discrimination in 2-D imaging.

Citation Download Citation

Ekaterina Borisova, Lilia Plamenova, Momchil Keremedchiev, Borislav Vladimirov, and Latchezar Avramov "Endogenous and exogenous fluorescence of gastrointestinal tumors: initial clinical observations", Proc. SPIE 8770, 17th International School on Quantum Electronics: Laser Physics and Applications, 87701C (15 March 2013); https://doi.org/10.1117/12.2016389

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