Characterization of atherosclerotic arterial tissue using multimodal non-linear optical microscopy

Riccardo Cicchi; Christian Matthäus; Tobias Meyer; Annika Lattermann; Benjamin Dietzek; Bernhard R. Brehm; Jürgen Popp; Francesco S. Pavone

doi:10.1117/12.2030879

17 June 2013 Characterization of atherosclerotic arterial tissue using multimodal non-linear optical microscopy

Riccardo Cicchi, Christian Matthäus, Tobias Meyer, Annika Lattermann, Benjamin Dietzek, Bernhard R. Brehm, Jürgen Popp, Francesco S. Pavone

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Proceedings Volume 8797, Advanced Microscopy Techniques III; 87970D (2013) https://doi.org/10.1117/12.2030879
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Atherosclerosis is among the most widespread cardiovascular diseases and one of the leading cause of death in the Western World. Characterization of arterial tissue in atherosclerotic condition is extremely interesting from the diagnostic point of view. Routinely used diagnostic methods, such as histopathological examination, are limited to morphological analysis of the examined tissues, whereas an exhaustive characterization requires a morpho-functional approach. Non-linear microscopy techniques have the potential to bridge this gap by providing morpho-functional information in a label-free way. Here we employed multiple non-linear microscopy techniques, including CARS, TPF, and SHG to provide intrinsic optical contrast from various tissue components in both arterial wall and atherosclerotic plaques. CARS and TPF microscopy were used to respectively image lipid depositions within plaques and elastin in the arterial wall. Cholesterol deposition in the lumen and collagen in the arterial wall were selectively imaged by SHG microscopy and distinguished by forward-backward SHG ratio. Image pattern analysis allowed characterizing collagen organization in different tissue regions. The presented method has the potential to find a stable place in clinical setting as well as to be applied in vivo in the near future.

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Riccardo Cicchi, Christian Matthäus, Tobias Meyer, Annika Lattermann, Benjamin Dietzek, Bernhard R. Brehm, Jürgen Popp, and Francesco S. Pavone "Characterization of atherosclerotic arterial tissue using multimodal non-linear optical microscopy", Proc. SPIE 8797, Advanced Microscopy Techniques III, 87970D (17 June 2013); https://doi.org/10.1117/12.2030879

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KEYWORDS

Second-harmonic generation

Collagen

Tissues

Microscopy

Signal detection

Image analysis

Tissue optics

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