Probe-based confocal laser endomicroscopy in head and neck malignancies: early preclinical experience

Anna Englhard; Susanne Girschick; Brigitte Mack; Veronika Volgger; Oliver Gires; Christian Conderman; Herbert Stepp; Christian Stephan Betz

doi:10.1117/12.2032628

24 June 2013 Probe-based confocal laser endomicroscopy in head and neck malignancies: early preclinical experience

Anna Englhard, Susanne Girschick, Brigitte Mack, Veronika Volgger, Oliver Gires, Christian Conderman, Herbert Stepp, Christian Stephan Betz

Author Affiliations +

Proceedings Volume 8805, Head and Neck Optical Diagnostics; 880506 (2013) https://doi.org/10.1117/12.2032628
Event: European Conferences on Biomedical Optics, 2013, Munich, Germany

Abstract

Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLEprobes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.

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Anna Englhard, Susanne Girschick, Brigitte Mack, Veronika Volgger, Oliver Gires, Christian Conderman, Herbert Stepp, and Christian Stephan Betz "Probe-based confocal laser endomicroscopy in head and neck malignancies: early preclinical experience", Proc. SPIE 8805, Head and Neck Optical Diagnostics, 880506 (24 June 2013); https://doi.org/10.1117/12.2032628

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KEYWORDS

Head

Neck

Confocal microscopy

Tissues

Biopsy

Endomicroscopy

Natural surfaces

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