In vitro therapeutic effect of PDT combined with VEGF-A gene therapy

Rumwald Leo G. Lecaros; Leaf Huang; Yih-Chih Hsu

doi:10.1117/12.2042979

27 February 2014 In vitro therapeutic effect of PDT combined with VEGF-A gene therapy

Rumwald Leo G. Lecaros, Leaf Huang, Yih-Chih Hsu

Proceedings Volume 8944, Biophotonics and Immune Responses IX; 894405 (2014) https://doi.org/10.1117/12.2042979
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Vascular endothelial growth factor A (VEGF-A), commonly known as VEGF, is one of the primary factors that affect tumor angiogenesis. It was found to be expressed in cancer cell lines including oral squamous cell carcinoma. Photodynamic therapy (PDT) is a novel therapeutic modality to treat cancer by using a photosensitizer which is activated by a light source to produce reactive oxygen species and mediates oxygen-independent hypoxic conditions to tumor. Another emerging treatment to cure cancer is the use of interference RNA (e.g. siRNA) to silence a specific mRNA sequence. VEGF-A was found to be expressed in oral squamous cell carcinoma and overexpressed after 24 hour post-PDT by Western blot analysis. Cell viability was found to decrease at 25 nM of transfected VEGF-A siRNA. In vitro combined therapy of PDT and VEGF-A siRNA showed better response as compared with PDT and gene therapy alone. The results suggest that PDT combined with targeted gene therapy has a potential mean to achieve better therapeutic outcome.

Citation Download Citation

Rumwald Leo G. Lecaros, Leaf Huang, and Yih-Chih Hsu "In vitro therapeutic effect of PDT combined with VEGF-A gene therapy", Proc. SPIE 8944, Biophotonics and Immune Responses IX, 894405 (27 February 2014); https://doi.org/10.1117/12.2042979

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