Full field photothermal dynamics microscopy

Guichen Tang; Fanting Kong; Y. C. Chen; M. Xu

doi:10.1117/12.2042326

12 March 2014 Full field photothermal dynamics microscopy

Guichen Tang, Fanting Kong, Y. C. Chen, M. Xu

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 89490X (2014) https://doi.org/10.1117/12.2042326
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present here a novel full field pump-probe photothermal dynamics microscopy (PTDM) which uses a numerical lockin mechanism for capturing full field photothermal responses and is capable of imaging 2D thermal dissipation dynamics by varying the time delay between the probing and pump nano-second pulses. PTDM may find interesting applications in biology and medicine. As one example, we report PTDM imaging nuclei contained on intact hematoxylin and eosin (H&E) stained prostate cancer specimens may potentially be used to distinguish low grade and high grade prostate cancer.

Citation Download Citation

Guichen Tang, Fanting Kong, Y. C. Chen, and M. Xu "Full field photothermal dynamics microscopy", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 89490X (12 March 2014); https://doi.org/10.1117/12.2042326

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available