Characterization of BxPC3-transplanted mice by hyperspectral autofluorescence imaging and Raman spectroscopy

Masanori Sawa; Bibin Bintang Andriana; Hidetoshi Sato

doi:10.1117/12.2039271

28 February 2014 Characterization of BxPC3-transplanted mice by hyperspectral autofluorescence imaging and Raman spectroscopy

Masanori Sawa, Bibin Bintang Andriana, Hidetoshi Sato

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Proceedings Volume 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics; 89510S (2014) https://doi.org/10.1117/12.2039271
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Live subcutaneous tumor grown in nude mouse is studied in situ with hyperspectral autofluorescence imaging and Raman spectroscopy. The purpose of the study is to develop methods for characterization of biochemical changing and of histological type of tumor without labeling. The results show that there are site depending variation in the fluorescence and Raman spectra. At the spot in which calcification is in process, Raman spectra showed a strong and specific band at 957 cm^-1 due to PO₄ species. The autofluosescence image can prove the histological changes based on the NADH and FAD which are major fluorophores in biological tissues. The hyperspectral image is analyzed with principal component analysis and the reconstructed images successfully depicts a different between necrotic and viable part within living subcutaneous tumor.

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Masanori Sawa, Bibin Bintang Andriana, and Hidetoshi Sato "Characterization of BxPC3-transplanted mice by hyperspectral autofluorescence imaging and Raman spectroscopy", Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 89510S (28 February 2014); https://doi.org/10.1117/12.2039271

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KEYWORDS

Tissues

Raman spectroscopy

Tumors

Auto-fluorescence imaging

Cancer

Hyperspectral imaging

Image filtering

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