Development Of A Time-Resolved Microfluorimeter With A Synchroscan Streak Camera And Its Application To Studies Of Cell Membranes

Akihiro Kusurni; Akihiko Tsuji; Masayuki Murata; Yasushi Sako; Akiyasu C Yoshizawa; Tsuyoshi Hayakawa; Shun-ichi Ohnishi

doi:10.1117/12.945412

24 June 1988 Development Of A Time-Resolved Microfluorimeter With A Synchroscan Streak Camera And Its Application To Studies Of Cell Membranes

Akihiro Kusurni, Akihiko Tsuji, Masayuki Murata, Yasushi Sako, Akiyasu C Yoshizawa, Tsuyoshi Hayakawa, Shun-ichi Ohnishi

Author Affiliations +

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945412
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

A time-resolved microfluorimeter with a synchroscan streak camera, which can be extended to a fluorescence lifetime imaging microscope (FLIM), has been developed for the use in biomedical research and clinical diagnosis. The schematic diagram of the instrument is given in Figure 1. The key idea for this instrument is to acquire molecular information on the structure of interest observed under the fluorescence microscope by using time-resolved fluorescence spectroscopy. The fluorescence lifetime is probably the only physical observable, the absolute value of which can be reliably determined in fluorescence microscopy.

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Akihiro Kusurni, Akihiko Tsuji, Masayuki Murata, Yasushi Sako, Akiyasu C Yoshizawa, Tsuyoshi Hayakawa, and Shun-ichi Ohnishi "Development Of A Time-Resolved Microfluorimeter With A Synchroscan Streak Camera And Its Application To Studies Of Cell Membranes", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945412

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