Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe

Riccardo Cicchi; Suresh Anand; Susanna Rossari; Alessandro Sturiale; Flavio Giordano; Vincenzo De Giorgi; Vincenza Maio; Daniela Massi; Gabriella Nesi; Anna Maria Buccoliero; Francesco Tonelli; Renzo Guerrini; Nicola Pimpinelli; Francesco Saverio Pavone

doi:10.1117/12.2052197

8 May 2014 Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe

Riccardo Cicchi, Suresh Anand, Susanna Rossari, Alessandro Sturiale, Flavio Giordano, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Francesco Tonelli, Renzo Guerrini, Nicola Pimpinelli, Francesco Saverio Pavone,

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 912932 (2014) https://doi.org/10.1117/12.2052197
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

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Riccardo Cicchi, Suresh Anand, Susanna Rossari, Alessandro Sturiale, Flavio Giordano, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Francesco Tonelli, Renzo Guerrini, Nicola Pimpinelli, Francesco Saverio Pavone, and "Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 912932 (8 May 2014); https://doi.org/10.1117/12.2052197

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