Diffraction computed tomography reveals the inner structure of complex biominerals

Hanna Leemreize; Mie Birkbak; Simon Frølich; Peter Kenesei; Jonathan D. Almer; Stuart R. Stock; Henrik Birkedal

doi:10.1117/12.2061580

11 September 2014 Diffraction computed tomography reveals the inner structure of complex biominerals

Hanna Leemreize, Mie Birkbak, Simon Frølich, Peter Kenesei, Jonathan D. Almer, Stuart R. Stock, Henrik Birkedal

Proceedings Volume 9212, Developments in X-Ray Tomography IX; 92120C (2014) https://doi.org/10.1117/12.2061580
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

Biological materials are complex and their investigation demands advanced characterization tools capable of elucidating their structure in three dimensions without the need for complicated sample preparation. Herein, we discuss our implementation of diffraction/scattering computed tomography (DSCT). DSCT is based on the use of diffraction information for tomographic reconstructions rather than linear attenuation as in regular μ-CT. This provides much additional information on the material under investigation. We illustrate the use of DSCT by discussion of data on a biomineralized attachment organ from a marine mussel. DSCT allowed mapping the spatial distribution of calcium carbonate polymorphs aragonite and calcite even though they were indistinguishable in absorption tomography. Detailed analysis of reconstructed diffraction patterns may provide additional insights as exemplified in the present case by mapping of the degree of chemical substitution in calcite.

Citation Download Citation

Hanna Leemreize, Mie Birkbak, Simon Frølich, Peter Kenesei, Jonathan D. Almer, Stuart R. Stock, and Henrik Birkedal "Diffraction computed tomography reveals the inner structure of complex biominerals", Proc. SPIE 9212, Developments in X-Ray Tomography IX, 92120C (11 September 2014); https://doi.org/10.1117/12.2061580

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available