Differentiation of bacterial colonies and temporal growth patterns using hyperspectral imaging

Mehrube Mehrübeoglu; Gregory W. Buck; Daniel W. Livingston

doi:10.1117/12.2063601

19 September 2014 Differentiation of bacterial colonies and temporal growth patterns using hyperspectral imaging

Mehrube Mehrübeoglu, Gregory W. Buck, Daniel W. Livingston

Proceedings Volume 9222, Imaging Spectrometry XIX; 922206 (2014) https://doi.org/10.1117/12.2063601
Event: SPIE Optical Engineering + Applications, 2014, San Diego, California, United States

Abstract

Detection and identification of bacteria are important for health and safety. Hyperspectral imaging offers the potential to capture unique spectral patterns and spatial information from bacteria which can then be used to detect and differentiate bacterial species. Here, hyperspectral imaging has been used to characterize different bacterial colonies and investigate their growth over time. Six bacterial species (Pseudomonas fluorescens, Escherichia coli, Serratia marcescens, Salmonella enterica, Staphylococcus aureus, Enterobacter aerogenes) were grown on tryptic soy agar plates. Hyperspectral data were acquired immediately after, 24 hours after, and 96 hours after incubation. Spectral signatures from bacterial colonies demonstrated repeatable measurements for five out of six species. Spatial variations as well as changes in spectral signatures were observed across temporal measurements within and among species at multiple wavelengths due to strengthening or weakening reflectance signals from growing bacterial colonies based on their pigmentation. Between-class differences and within-class similarities were the most prominent in hyperspectral data collected 96 hours after incubation.

Citation Download Citation

Mehrube Mehrübeoglu, Gregory W. Buck, and Daniel W. Livingston "Differentiation of bacterial colonies and temporal growth patterns using hyperspectral imaging", Proc. SPIE 9222, Imaging Spectrometry XIX, 922206 (19 September 2014); https://doi.org/10.1117/12.2063601

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