Subcellular imaging of mechanical and chemical properties using Brillouin and Raman microspectroscopies

Zhaokai Meng; Vladislav V. Yakovlev

doi:10.1117/12.2080317

13 April 2015 Subcellular imaging of mechanical and chemical properties using Brillouin and Raman microspectroscopies

Zhaokai Meng, Vladislav V. Yakovlev

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93280P (2015) https://doi.org/10.1117/12.2080317
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Brillouin and Raman microspectroscopies are powerful techniques for noninvasive optical imaging. In particular, Brillouin microscopy is a unique technique, which allows assessing sample’s mechanical properties with optical level spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image turbid samples without substantial degradation of the data quality. In this report, we applied Brillouin spectroscopy to imaging individual cells. These viscoelastic measurements were supplemented by chemically specific measurements using confocal Raman microspectroscopy.

Citation Download Citation

Zhaokai Meng and Vladislav V. Yakovlev "Subcellular imaging of mechanical and chemical properties using Brillouin and Raman microspectroscopies", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280P (13 April 2015); https://doi.org/10.1117/12.2080317

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