A rapid detection method of Escherichia coli by surface enhanced Raman scattering

Feifei Tao; Yankun Peng; Tianfeng Xu

doi:10.1117/12.2176826

13 May 2015 A rapid detection method of Escherichia coli by surface enhanced Raman scattering

Feifei Tao, Yankun Peng, Tianfeng Xu

Proceedings Volume 9488, Sensing for Agriculture and Food Quality and Safety VII; 94880Q (2015) https://doi.org/10.1117/12.2176826
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

Conventional microbiological detection and enumeration methods are time-consuming, labor-intensive, and giving retrospective information. The objectives of the present work are to study the capability of surface enhanced Raman scattering (SERS) to detect Escherichia coli (E. coli) using the presented silver colloidal substrate. The obtained results showed that the adaptive iteratively reweighed Penalized Least Squares (airPLS) algorithm could effectively remove the fluorescent background from original Raman spectra, and Raman characteristic peaks of 558, 682, 726, 1128, 1210 and 1328 cm^-1 could be observed stably in the baseline corrected SERS spectra of all studied bacterial concentrations. The detection limit of SERS could be determined to be as low as 0.73 log CFU/ml for E. coli with the prepared silver colloidal substrate. The quantitative prediction results using the intensity values of characteristic peaks were not good, with the correlation coefficients of calibration set and cross validation set of 0.99 and 0.64, respectively.

Citation Download Citation

Feifei Tao, Yankun Peng, and Tianfeng Xu "A rapid detection method of Escherichia coli by surface enhanced Raman scattering", Proc. SPIE 9488, Sensing for Agriculture and Food Quality and Safety VII, 94880Q (13 May 2015); https://doi.org/10.1117/12.2176826

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