Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

David Dannhauser; Pasquale Memmolo; Domenico Rossi; Francesco Merola; Lisa Miccio; Filippo Causa; Pietro Ferraro; Paolo A. Netti

doi:10.1117/12.2185113

22 June 2015 Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

David Dannhauser, Pasquale Memmolo, Domenico Rossi, Francesco Merola, Lisa Miccio, Filippo Causa, Pietro Ferraro, Paolo A. Netti

Author Affiliations +

Proceedings Volume 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II; 95290D (2015) https://doi.org/10.1117/12.2185113
Event: SPIE Optical Metrology, 2015, Munich, Germany

Abstract

The Light Scattering Profile (LSP) of an individual cell provides a fast and accurate characterization of its morphological properties. By combining a camera-based small angle light scattering apparatus with a microfluidic-induced particle migration technique, it is possible to characterize cells in microfluidic flows. The scattering profile of an individual cell can be fully characterized by our optimized optical light collection system. Viscoelastic-induced particle migration by polyethylene oxide implemented in a low-cost microfluidic device composed of an alignment section and a measuring section opens the possibility of precise, label-free, individual cell analysis. We have studied living cells in microfluidic flows by our light scattering apparatus and by a Digital Holographic Microscope (DHM) system. Our DHM measurements provided an accurate 3D position tracking even in multiple cell conditions.

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David Dannhauser, Pasquale Memmolo, Domenico Rossi, Francesco Merola, Lisa Miccio, Filippo Causa, Pietro Ferraro, and Paolo A. Netti "Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique", Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95290D (22 June 2015); https://doi.org/10.1117/12.2185113

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