Heating drug delivery to vascular wall with Rhodamine B and fluorescence labeled Paclitaxel ranging 50 to 70°C: ex vivo study

R. Homma; M. Shinozuka; N. Shimazaki; T. Arai

doi:10.1117/12.2210780

7 March 2016 Heating drug delivery to vascular wall with Rhodamine B and fluorescence labeled Paclitaxel ranging 50 to 70°C: ex vivo study

R. Homma, M. Shinozuka, N. Shimazaki, T. Arai

Author Affiliations +

Proceedings Volume 9706, Optical Interactions with Tissue and Cells XXVII; 97060G (2016) https://doi.org/10.1117/12.2210780
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We studied heating drug delivery to vascular wall with Rhodamine B ranging 50 to 70°C ex vivo study. Porcine carotid artery was dipped in the heated Rhodamine B solution in 15 s and then cooled by 37°C saline. Rhodamine B concentration distribution in the vascular wall cross-section was measured by a fluorescence microscope using 550 nm for excitation and 620 nm emission for fluorescence detection. The total amount of measured fluorescence in the vascular wall was calculated as a indication of delivered Rhodamine B quantity. The delivered Rhodamine B quantity was increased with increasing heating temperature with 50 to 70°C. In the cases of 60 to 70°C heating, the delivered Rhodamine B quantity was 3.1 to 23.3 fold by that of 37°C. Defined penetration depth of the delivered Rhodamine B in the vascular wall was also significantly increased with 65°C and 70°C heating. We also studied heating drug delivery to the vascular wall with fluorescence labeled Paclitaxel with 70°C in 15 s and 60 s heating ex vivo. In both contact duration, the delivered Paclitaxel quantity was increased. To understand these drug delivery enhancement effects, we investigated the vascular cross-sectional structure change by the heating. Some holes over 50 nm in diameter appeared on the internal elastic lamina with 70°C heating. We prospected that vascular surface structure change by the heating might enhance drug delivery to the vascular wall.

Citation Download Citation

R. Homma, M. Shinozuka, N. Shimazaki, and T. Arai "Heating drug delivery to vascular wall with Rhodamine B and fluorescence labeled Paclitaxel ranging 50 to 70°C: ex vivo study", Proc. SPIE 9706, Optical Interactions with Tissue and Cells XXVII, 97060G (7 March 2016); https://doi.org/10.1117/12.2210780

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