Rapid label-free chemical imaging of cells and tissues
(Conference Presentation)

Marcus T. Cicerone; Charles H. Camp Jr.

doi:10.1117/12.2216118

27 April 2016 Rapid label-free chemical imaging of cells and tissues (Conference Presentation)

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 97120C (2016) https://doi.org/10.1117/12.2216118
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Coherent Raman imaging methods have been under development for almost 15 years. The field is beginning to mature, transitioning from a “new techniques” phase to an applications phase. I will discuss current capabilities of broadband coherent anti-Stokes Raman scattering (BCARS) microscopy using optimized excitation paradigms, and provide a few examples of how broadband BCARS imaging has helped to answer (or raise) questions in investigations of tissues and small organisms. I will also discuss progress in processing BCARS spectra to make them independent of excitation profile or non-resonant response, and directly comparable to spontaneous Raman spectra. I will also discuss progress on a new approach to time-domain BCARS that promises to significantly simplify and speed BCARS data acquisition.

Conference Presentation

Citation Download Citation

Marcus T. Cicerone and Charles H. Camp Jr. "Rapid label-free chemical imaging of cells and tissues (Conference Presentation)", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97120C (27 April 2016); https://doi.org/10.1117/12.2216118

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