Size dependent gold nanoparticle interaction at nano-micro interface using both monolayer and multilayer (tissue-like) cell models

Darren Yohan; Celina Yang; Xiaofeng Lu; Devika B. Chithrani

doi:10.1117/12.2217987

22 April 2016 Size dependent gold nanoparticle interaction at nano-micro interface using both monolayer and multilayer (tissue-like) cell models

Darren Yohan, Celina Yang, Xiaofeng Lu, Devika B. Chithrani

Author Affiliations +

Proceedings Volume 9722, Colloidal Nanoparticles for Biomedical Applications XI; 97220T (2016) https://doi.org/10.1117/12.2217987
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Gold nanoparticles (GNPs) can be used as a model NP system to improve the interface between nanotechnology and medicine since their size and surface properties can be tailored easily. GNPs are being used as radiation dose enhancers and as drug carriers in cancer research. Hence, it is important to know the optimum NP size for uptake not only at monolayer level but also at tissue level. Once GNPs leave tumor vasculature, they enter the tumor tissue. Success of any therapeutic technique using NPs depends on how well NPs penetrate the tumor tissue and reach individual tumor cells. In this work, multicellular layers (MCLs) were grown to model the post-vascular tumor environment. GNPs of 20 nm and 50 nm diameters were used to elucidate the effects of size on the GNP penetration and distribution dynamics. Larger NPs (50 nm) were better at monolayer level, but smaller NPs (20 nm) were at tissue level. The MCLs exhibited a much more extensive extracellular matrix (ECM) than monolayer cell cultures. This increased ECM created a barrier for NP transport and ECM was also dependent on the tumor cell lines. Smaller NPs penetrated better compared to larger NPs. Transport of NPs was better in MDA-MB231 vs MCF-7. This MCL model tissue structures are better tools to optimize NP transport through tissue before using them in animal models. Based on our study, we believe that smaller NPs are better for improved outcome in future cancer therapeutics.

Citation Download Citation

Darren Yohan, Celina Yang, Xiaofeng Lu, and Devika B. Chithrani "Size dependent gold nanoparticle interaction at nano-micro interface using both monolayer and multilayer (tissue-like) cell models", Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97220T (22 April 2016); https://doi.org/10.1117/12.2217987

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