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28 April 2022 Prism nerve holder for enhanced OCT volumetric imaging of peripheral nerves
Ilyas Saytashev, Anthony Parra, Yong-Chul Yoon, Benjamin J. Vakoc, Srikanth Vasudevan, Daniel X. Hammer
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Proceedings Volume PC11946, Neural Imaging and Sensing 2022; PC119460D (2022) https://doi.org/10.1117/12.2610135
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We have developed a novel nerve holder, imaging protocol and post-processing routine which enhance OCT imaging by accessing peripheral nerve from 3 sides. Two mirror prisms provide imaging access from sides of a peripheral nerve in addition to conventional volume acquisition from the top. Prism glass compensates for changed focusing and optical pathlength during nerve imaging from its sides Acquired prism sub-volumes and top-bottom imaged nerve sub-volume are merged in two-stage semi-automated postprocessing routine. We demonstrate successful application of this approach on tissue-mimicking phantoms, as well as in vivo rat sciatic nerve imaging.
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Ilyas Saytashev, Anthony Parra, Yong-Chul Yoon, Benjamin J. Vakoc, Srikanth Vasudevan, and Daniel X. Hammer "Prism nerve holder for enhanced OCT volumetric imaging of peripheral nerves", Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC119460D (28 April 2022); https://doi.org/10.1117/12.2610135
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KEYWORDS
Nerve
Optical coherence tomography
Prisms
In vivo imaging
Tissue optics
Electrodes
Epilepsy
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