Interferometric phase and absorption microscopy (iPAM) for sickle cell disease imaging (Conference Presentation)

Alex C. Matlock; Yuhao Qiang; Ming Dao; John Higgins; Zahid Yaqoob; Peter So

doi:10.1117/12.2648428

16 March 2023 Interferometric phase and absorption microscopy (iPAM) for sickle cell disease imaging (Conference Presentation)

Alex C. Matlock, Yuhao Qiang, Ming Dao, John Higgins, Zahid Yaqoob, Peter So

Author Affiliations +

Proceedings Volume PC12389, Quantitative Phase Imaging IX; PC123890R (2023) https://doi.org/10.1117/12.2648428
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present the interferometric phase and absorption microscope (iPAM) platform for studying the biomechanics and fitness of sickle red blood cells (sRBCs). iPAM combines diffraction phase microscopy, tailored imaging media with customized optical properties, and extinction-based models to extract sRBC hemoglobin concentration and 3D structure from hundreds of cells in a single snapshot. Coupled with a customized microfluidic platform controlling cell deformation and oxygenation, we show that iPAM can provide a fitness index evaluating sRBC health that could act as an indicator detecting vaso-occlusive crisis risk and determine sickle cell disease treatment effectiveness.

Conference Presentation

Citation Download Citation

Alex C. Matlock, Yuhao Qiang, Ming Dao, John Higgins, Zahid Yaqoob, and Peter So "Interferometric phase and absorption microscopy (iPAM) for sickle cell disease imaging (Conference Presentation)", Proc. SPIE PC12389, Quantitative Phase Imaging IX, PC123890R (16 March 2023); https://doi.org/10.1117/12.2648428

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