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12 August 2023 Three-dimensional quantitative phase imaging: from label-free imaging of live cells to dielectric tensor tomography of birefringent materials
YongKeun Park
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Proceedings Volume PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; PC1262204 (2023) https://doi.org/10.1117/12.2679803
Event: SPIE Optical Metrology, 2023, Munich, Germany
Abstract
Quantitative phase imaging (QPI) is a powerful label-free imaging technique that enables high-resolution, three-dimensional imaging of unlabeled samples by exploiting refractive index (RI) distributions as intrinsic imaging contrast. In this talk, we present the latest developments in 3D QPI techniques for investigations of live cells, tissues, and organoids over a long period of time. We will introduce several reference-free QPI approaches with incoherent light sources and discuss the considerations of multiple light scattering into a tomographic reconstruction algorithm1-3. Additionally, we will present a method of measuring 3D dielectric tensor tomograms, which expands the applicability of QPI to 3D birefringent materials. These technological developments hold great promise for advancing 3D label-free high-resolution imaging and its application in various fields, particularly in photonics.
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YongKeun Park "Three-dimensional quantitative phase imaging: from label-free imaging of live cells to dielectric tensor tomography of birefringent materials", Proc. SPIE PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, PC1262204 (12 August 2023); https://doi.org/10.1117/12.2679803
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KEYWORDS
3D image processing
Tomography
Dielectrics
Phase imaging
Live cell imaging
Photonics
Reconstruction algorithms
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