A  miniaturized biosensor based on organic optoelectronics for fast and multiplexing plasmonic detection of high- and low-molecular weight analytes

Stefano Toffanin

doi:10.1117/12.2683963

17 October 2023 A miniaturized biosensor based on organic optoelectronics for fast and multiplexing plasmonic detection of high- and low-molecular weight analytes

Stefano Toffanin

Author Affiliations +

Proceedings Volume PC12741, Advanced Materials, Biomaterials, and Manufacturing Technologies for Security and Defence; PC1274109 (2023) https://doi.org/10.1117/12.2683963
Event: SPIE Security + Defence, 2023, Amsterdam, Netherlands

Abstract

Optical biosensors based on plasmonic sensing schemes combine high sensitivity and selectivity with label-free detection. However, the use of bulky optical components is still hampering the possibility of obtaining miniaturized systems required for analysis in real settings [1]. Here, we demonstrate a fully miniaturized optical biosensor prototype based on plasmonic detection that enables fast and multiplex sensing of analytes with high- and low-molecular weight (80000 and 582 Dalton) as quality and safety parameters for milk: a protein (lactoferrin) and an antibiotic (streptomycin). The optical sensor is based on the smart integration of (i) miniaturized organic optoelectronic devices used as light-emitting and -sensing elements and (ii) a functionalized nanostructured plasmonic grating for highly sensitive and specific localized surface plasmon resonance (SPR) detection [2]. The sensor provides quantitative and linear response reaching a limit of detection of 10^-4 refractive index units once it is calibrated by standard solutions. Analyte-specific and rapid (15 minute-long) immunoassay-based detection is demonstrated for both targets. By using a custom algorithm based on principal-component analysis, a linear dose-response curve is constructed which correlated with a limit of detection (LOD) as low as 3.7 μg/mL for lactoferrin, thus assessing that the miniaturized optical biosensor well-aligned with the reference benchtop SPR method [3].

Conference Presentation

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Stefano Toffanin "A miniaturized biosensor based on organic optoelectronics for fast and multiplexing plasmonic detection of high- and low-molecular weight analytes", Proc. SPIE PC12741, Advanced Materials, Biomaterials, and Manufacturing Technologies for Security and Defence, PC1274109 (17 October 2023); https://doi.org/10.1117/12.2683963

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KEYWORDS

Miniaturization

Plasmonics

Biological research

Organic optoelectronics

Biosensors

Multiplexing

Optical biosensors

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