Antibiotic susceptibility testing by infrared multispectral imaging : identification of optimal wavelengths

Stéphan Pissot; Mathieu Dupoy; Pierre R. Marcoux

doi:10.1117/12.3001548

13 March 2024 Antibiotic susceptibility testing by infrared multispectral imaging : identification of optimal wavelengths

Stéphan Pissot, Mathieu Dupoy, Pierre R. Marcoux

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Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC128460E (2024) https://doi.org/10.1117/12.3001548
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Antibiotic resistance is a critical public health concern requiring fast, affordable, and reliable diagnostic methods. This study focuses on identifying optimal wavelengths for multispectral imaging in antibiotic susceptibility testing. Deuterium isotope probing and FTIR spectroscopy were used to analyze the metabolic impact of antibiotics on bacteria. Characteristic wavelengths indicating variations in bacterial metabolism were identified. This approach holds promise for expedited antibiotic sensitivity assessment, potentially delivering results within two hours. The utilization of multispectral imaging presents a cost-effective and innovative tool for bacterial identification and combating antibiotic resistance.

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Stéphan Pissot, Mathieu Dupoy, and Pierre R. Marcoux "Antibiotic susceptibility testing by infrared multispectral imaging : identification of optimal wavelengths", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC128460E (13 March 2024); https://doi.org/10.1117/12.3001548

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KEYWORDS

Multispectral imaging

Infrared imaging

Infrared radiation

FT-IR spectroscopy

Bacteria

Spectroscopy

Mode conditioning cables

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