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13 March 2024 Luminosa: making confocal fluorescence microscopy a tool for every biophysicist
Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, Rainer Erdmann
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284710 (2024) https://doi.org/10.1117/12.3006371
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Quantitative time-resolved fluorescence techniques like FLIM are increasingly employed in fields like phase separation and cellular sensing. PicoQuant`s new microscope Luminosa combines state-of-the-art hardware with cutting edge software, delivering high quality data while simplifying daily operation. The software includes features like context-based workflows, sample-free auto-alignment and laser power calibration which improve reproducibility of experiments. We describe how FLIM is streamlined with Luminosa. Its rapidFLIM hardware records several frames per second with high count rates, which the software handles with a novel dynamic binning format. Combined with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images with minimal user interaction. We will also show an outlook about how Luminosa can be used for combining FLIM with super-resolution modalities.
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Felix Koberling, Fabio Barachati, Marcelle Koenig, Maria Loidolt-Krueger, Ellen Schmeyer, Matthias Patting, Marcus Sackrow, Uwe Ortmann, Evangelos Sisamakis, and Rainer Erdmann "Luminosa: making confocal fluorescence microscopy a tool for every biophysicist", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284710 (13 March 2024); https://doi.org/10.1117/12.3006371
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KEYWORDS
Confocal microscopy
Fluorescence lifetime imaging
Fluorescence microscopy
Image analysis
Statistical analysis
Photon counting
Process modeling
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