Label-free visualization of complex fiber structures in oral tissue using computational scattered light imaging

Hamed Abbasi; Sjors A. Koppes; Stijn Keereweer; Miriam Menzel

doi:10.1117/12.3002180

13 March 2024 Label-free visualization of complex fiber structures in oral tissue using computational scattered light imaging

Hamed Abbasi, Sjors A. Koppes, Stijn Keereweer, Miriam Menzel

Author Affiliations +

Proceedings Volume PC12856, Biomedical Applications of Light Scattering XIV; PC128560C (2024) https://doi.org/10.1117/12.3002180
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Computational Scattered Light Imaging (ComSLI) is a novel, non-destructive, whole-slide imaging method with the unique ability to precisely disentangle densely interwoven fiber structures in biological tissues. ComSLI can be performed on microscopy slides regularly prepared within the histopathological routine. Although it is a label-free method and does not require any staining, it also works on stained tissues for various stains. So far, ComSLI has been used to visualize nerve fibers in brain tissues [1-3]. In this study, we visualize muscle and collagen fibers in oral tissues for the first time. REFERENCES: [1] Menzel, M., et al. "Using light and X-ray scattering to untangle complex neuronal orientations and validate diffusion MRI." Elife 12 (2023). [2] Menzel, M., et al. "Scattered Light Imaging: Resolving the substructure of nerve fiber crossings in whole brain sections with micrometer resolution." NeuroImage 233 (2021). [3] Menzel, M., et al. "Toward a high-resolution reconstruction of 3D nerve fiber architectures and crossings in the brain using light scattering measurements and finite-difference time-domain simulations." Physical Review X 10.2 (2020).

Conference Presentation

Citation Download Citation

Hamed Abbasi, Sjors A. Koppes, Stijn Keereweer, and Miriam Menzel "Label-free visualization of complex fiber structures in oral tissue using computational scattered light imaging", Proc. SPIE PC12856, Biomedical Applications of Light Scattering XIV, PC128560C (13 March 2024); https://doi.org/10.1117/12.3002180

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