Detection of bound residues in soils by sandwich-immunoassay

M. Dosch; Michael G. Weller; Reinhard Niessner

doi:10.1117/12.224093

9 October 1995 Detection of bound residues in soils by sandwich-immunoassay

M. Dosch, Michael G. Weller, Reinhard Niessner

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224093
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

Immunoassays are useful analytical instruments for the detection of many environmental compounds. This method is now introduced for the detection of non-extractable compounds in soil. So-called 'bound residues' consist of a soil component, e.g. humic acids, and an irreversibly bound pollutant. Because of the complexity of those macromolecules conventional analytical methods in general do not work. Enzyme immunoassays, in contrast, seem to have a large potential for applications and further developments in this field. The use of antibodies with high affinity to the analytes makes a selective detection of environmental pollutants possible. With the development of an enzyme-labeled sandwich-immunoassay polycyclic aromatic hydrocarbons (PAHs), irreversibly bound to humic acids, were determined for the first time.

Citation Download Citation

M. Dosch, Michael G. Weller, and Reinhard Niessner "Detection of bound residues in soils by sandwich-immunoassay", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224093

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