Detection of the coliform bacteria Escherichia coli and Salmonella sp. in water by a sensitive and rapid immunomagnetic electrochemiluminescence (ECL) technique

H. Yu; J. Bruno

doi:10.1117/12.224106

9 October 1995 Detection of the coliform bacteria Escherichia coli and Salmonella sp. in water by a sensitive and rapid immunomagnetic electrochemiluminescence (ECL) technique

H. Yu, J. Bruno

Author Affiliations +

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224106
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

Hemorrhagic Escherichia coli O157:H7 and other fecal coliform bacteria, such as species of Salmonella, could pose a serious health threat in contaminated water resources. Traditional bacterial culture methods and ELISA based assays for identification of fecal coliforms are relatively slow and ambiguous. Polymerase chain reaction of extracted DNA from such bacteria and immunomagnetic separation (IMS) methods appear promising for this application. Although PCR can be a definitive identification technique, it is relatively time consuming when compared to IMS. In this work, the IMS technique has been coupled with an electrochemiluminescence (ECL) technology to separate specific bacteria from their media and quantitatively detect the bacteria within one hour. The sensitivity of the IMS-ECL assay for E.coli O157 strain and Salmonella sp. is as low as 10 - 100 cells/mL in water samples. In addition, IMS was accomplished in dense washings of food and environmental samples followed by ECL assay. These results suggest strongly use of the IMS-ECL methodology for rapid and facile screening of various bacterial contaminations in water resources or other environmental samples for the low level presence of pathogenic coliforms.

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H. Yu and J. Bruno "Detection of the coliform bacteria Escherichia coli and Salmonella sp. in water by a sensitive and rapid immunomagnetic electrochemiluminescence (ECL) technique", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224106

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