Fluorescence and reflectance spectra specify intrinsic fluorescence spectrum corrected for tissue optics distortion

Craig M. Gardner; Steven L. Jacques; Ashley J. Welch

doi:10.1117/12.144703

18 May 1993 Fluorescence and reflectance spectra specify intrinsic fluorescence spectrum corrected for tissue optics distortion

Craig M. Gardner, Steven L. Jacques, Ashley J. Welch

Author Affiliations +

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144703
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The dependence of total fluorescence escape from a homogeneous, semi-infinite tissue on tissue optics has been examined. A measurement of total fluorescence escape (F) is related to the intrinsic fluorescence coefficient ((Beta) ) by a factor that depends on the optical properties of the tissue: F((lambda) ) equals (Beta) ((lambda) _x, (lambda) ) (integral) _O^(infinity ) (Phi) (z; (lambda) _x) E(z; (lambda) ) dz. (Beta) is the product of fluorophore absorption coefficient and fluorophore quantum yield. (Phi) is the fluence rate of excitation light ((lambda) _x) in the tissue. E is a dimensionless function that describes the probability for escape to the surface of emission light ((lambda) ) originating at a depth z. The source strength of fluorescence emission at depth z is the product (Beta) (Phi) . Accurate expressions have been developed for the depth dependence of (Phi) and E, valid for both absorption-dominant and scattering-dominant light wavelengths in a tissue. These expressions have been based on Monte Carlo simulations of photon transport. They have been used to evaluate the integral in the above equation to yield an analytic expression for fluorescence escape which is a function of the total diffuse reflectance (R_d) and optical penetration depth ((delta) ) at both the excitation and emission wavelengths. Tissue phantoms have been used to experimentally verify the theory of total fluorescence escape. Non-invasive measurements of total fluorescence escape, diffuse reflectance, and penetration depth allow determination of the intrinsic fluorescence spectrum.

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Craig M. Gardner, Steven L. Jacques, and Ashley J. Welch "Fluorescence and reflectance spectra specify intrinsic fluorescence spectrum corrected for tissue optics distortion", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144703

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