Temporal and spectral separation of singlet oxygen luminescence from near-infrared emitting photosensitizers

John R. Gilchrist; Dirk U. Nather

doi:10.1117/12.144726

18 May 1993 Temporal and spectral separation of singlet oxygen luminescence from near-infrared emitting photosensitizers

John R. Gilchrist, Dirk U. Nather

Author Affiliations +

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144726
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

The luminescence emission of singlet molecular oxygen (¹O₂) generated by bacteriopheophytin a, a near infrared emitting photosensitizer, was measured using a new high sensitivity spectrometer system for time and spectral resolved near infrared detection. The instrument uses a low energy pulsed nitrogen laser (40 (mu) J per pulse) to optically excite the photosensitizer and is capable of a time resolution of 40 ns per data point and an instrument response function of 350 ns FWHM (full width at half maximum). The use of a low energy (and relatively low cost) source provides sufficient system sensitivity to measure time resolved spectra in the near infrared with high spectral and temporal resolution. The simultaneous detection, with high accuracy and repeatability, of both the temporal and spectral dependence of the photo-processes of ¹O₂ generation, especially with near infrared emitting photosensitizers, may further stimulate the current intensive investigations concerning the activity of ¹O₂ to bio-molecules.

Citation Download Citation

John R. Gilchrist and Dirk U. Nather "Temporal and spectral separation of singlet oxygen luminescence from near-infrared emitting photosensitizers", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144726

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